P-33: Effect of Cell Phone Electromagnetic Fields on In vitro Maturation of Immature Mice Oocytes

Background: In recent years, the widespread use of microwave producing instruments specially mobile phones; result in growing concern regarding the possible effects associated with these waves on human health. Some studies highlight the possible health hazards of electromagnetic fields (EMF), while others contest the hypothesis of biological impact of EMF. The aim of this study was to evaluate the effect of electromagnetic fields emitted by a mobile phone on resumption of meiosis, in vitro maturation of immature mouse oocytes by resulting metaphase II oocytes. Materials and Methods: Germinal vesicles (GV) were obtained from female NMRI mice 46-48 hours after administration of an intra-peritoneal injection of 5 IU PMSG and cultured in Alpha MEM. Germinal vesicles were exposed to a ringing mobile set on silent tone placed in the Co2 incubator close to culture dish for 1 hour. After 24 hours, the number of metaphase ІІ (MІІ) oocytes was recorded under an invert microscope. The same condition was created for control group except the mobile exposure. Results: The percentage of matured M II oocytes in the control group (74 M II from 107 GVs), was significantly higher (p value=0.0424) than the experimental group (79 MII from 121 GVs). Conclusion: We figured out that exposure of mobile phone decrease the oocyte maturation of GV.